BUCHAREST, Romania — At first, fans in Romania politely applauded the Roma performers sharing a stage with Madonna. Then the pop star condemned widespread discrimination against Roma, or Gypsies — and the cheers gave way to jeers.

The sharp mood change that swept the crowd of 60,000, who had packed a Bucharest park for Wednesday night's concert, underscores how prejudice against Gypsies remains deeply entrenched across Eastern Europe.

Despite long-standing efforts to stamp out rampant bias, human rights advocates say Roma probably suffer more humiliation and endure more discrimination than any other people group on the continent.

Sometimes, it can be deadly: In neighboring Hungary, six Roma have been killed and several wounded in a recent series of apparently racially motivated attacks targeting small countryside villages predominantly settled by Gypsies.

While discrimination is widespread, many Eastern Europeans are enthusiastic about Gypsy music and dance, which they embrace as part of the region's cultural heritage.

That explains why the Roma musicians and a dancer who had briefly joined Madonna onstage got enthusiastic applause. And it also may explain why some in the crowd turned on Madonna when she paused during the two-hour show — a stop on her worldwide "Sticky and Sweet" tour — to touch on their plight.

"It has been brought to my attention … that there is a lot of discrimination against Romanies and Gypsies in general in Eastern Europe," she said. "It made me feel very sad."

Thousands booed and jeered her.

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A few cheered when she added: "We don't believe in discrimination … we believe in freedom and equal rights for everyone." But she got more boos when she mentioned discrimination against homosexuals and others.

"I jeered her because it seemed false what she was telling us. What business does she have telling us these things?" said Ionut Dinu, 23.

Madonna did not react and carried on with her concert, held near the hulking palace of the late communist dictator Nicolae Ceausescu.

Her publicist, Liz Rosenberg, said Madonna and others had told her there were cheers as well as jeers.

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