Well-wishers bid "happy trails" to the Roy Rogers-Dale Evans Museum in Victorville, Calif., which is moving to Branson, Mo.
The museum, which closed its doors Sunday, hopes to open in Branson for the Memorial Day weekend.
Rogers — known to Western movie fans as the "King of the Cowboys" — died in 1998 at age 86. Evans, who wrote the couple's theme song, "Happy Trails to You," died in 2001 at 88.
"We've been here 36 years in the high desert, so it's a little bit rough," said son Dusty Rogers.
He added: "We took mom to Branson about six years ago and she just loved it. Basically we're fulfilling what she wanted to do."
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Museum attendance — which had peaked in the 1990s at around 75,000 visitors a year — had fallen to fewer than 50,000, he said.